Figure 6.

Inhibiting proteasome activity impedes quiescence entry and increases drug sensitivity of the reactivated cells. (A) The reactivated cells were treated with or without indicative concentration of PTX (Left) or 100 µM Cariporide (Right) for 24 h. Ubiquitination of cellular proteins was detected by Western blot using anti-Ub antibody. (B-D) The reactivated cells were treated with higher dose of PTX (HepG2^Reac 160 nM, UMUC3^Reac 500nM) combined with or without 1 µM MG132 for 24 h, (B) ubiquitination of proteins was detected by WB, (C) cell proliferation was detected by EdU incorporation, *p<0.05, and (D) cells apoptosis was examined by flow cytometry.