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. 2020 Jun 15;17(11):1474–1481. doi: 10.7150/ijms.45831

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RSF induced sub‑G1 phase ratio increase and mitochondrial damage. (A) Cell cycle analysis was conducted by flow cytometry. (B) Sub-G1 fractions are expressed as percentages. (C) The fluorescence for mitochondrial depolarization assay was measured by FACS analysis. (D) The relative mitochondrial TMRM fluorescence levels were calculated to determine the fold difference in comparison to control. Results are presented as means ± SEMs. *p<0.05. **p<0.01. RSF, ethanolic extract of Radix Sophorae Flavescentis; CTRL, control.