(A). Immunoblot of dsRNA sensing pathway members in vector control or KAP1 knockout B16F10 cells 3 days post irradiation with sham or 8Gy X-rays.
(B) qRT-PCR quantification of interferon-stimulated genes in vector control or KAP1 knockout B16F10 cells 3 days post exposure to sham or 8Gy X-rays.
(C). Immunoblot of dsRNA sensing pathway members from KAP1/MAVS double knockout B16F10 cells 3 days after exposure to 8Gy X-rays.
Error bars represent SEM. *p,<0.05, **p<0.01, ***p<0.001, ns, not significant, as determined by unpaired Student’s T-test (B)