(A, B). Tumor growth (A) and Kaplan-Meier survival curves (B) of Balb/C mice inoculated with 5×105 vector control or KAP1 knockout 4T1 cells and treated with 0Gy or 8 Gy of X-rays at day 8 post inoculation.
(C). Representative images of lungs of mice inoculated vector control and KAP1 knockout 4T1 cells.
(D, E). Quantification of lung metastases by mass (D) and metastatic surface nodules (E) (n=5 per group).
Data represent two independent experiments (A and B). Error bars represent SEM. *p<0.05, **p<0.01, ***p<0.001, ns, not significant, as determined by two-way ANOVA (A), log-rank (B), or unpaired Student’s T-test (D and E)