Table 1:
RNA primers used for CRISPR/Cas9 and qPCR
| Gene Names | Species | Usage | Forward sequence | Reverse sequence (if applicable) |
|---|---|---|---|---|
| TRIM28/KAP1 | Human | sgRNA | GTTCGCATCCTGGGCGTCGG | |
| TRIM28/KAP1 | Human | sgRNA | AATTATTTCATGCGTGATAG | |
| TRIM28/KAP1 | Mouse | sgRNA | CGCCGCAGCGAATAATTCGG | |
| TRIM28/KAP1 | Mouse | sgRNA | TATGCGTGATAGTGGCAGTA | |
| MAVS | Mouse | sgRNA | GCCACCAGACATCCTCGCGA | |
| MAVS | Mouse | sgRNA | GAGGACAAACCTCTTGTCTG | |
| GAPDH | Human | qPCR | AGGGCTGCTTTTAACTCTGGT | CCCCACTTGATTTTGGAGGGA |
| MER21C | Human | qPCR | GGAGCTTCCTGATTGGCAGA | ATGTAGGGTGGCAAGCACTG |
| MER57B1 | Human | qPCR | CCTCCTGAGCCAGAGTAGGT | ACCAGTCTGGCTGTTTCTGT |
| MLT1C49 | Human | qPCR | TATTGCCGTACTGTGGGCTG | TGGAACAGAGCCCTTCCTTG |
| ACTB | Mouse | qPCR | GAAATCGTGCGTGACATCAAA | TGTAGTTTCATGGATGCCACA |
| IFIT1 | Mouse | qPCR | CAAGGCAGGTTTCTGAGGAG | GACCTGGTCACCATCAGCAT |
| CXCL10 | Mouse | qPCR | GCCGTCATTTTCTGCCTCA | CGTCCTTGCGAGAGGGATC |
| ISG15 | Mouse | qPCR | CTAGAGCTAGAGCCTGCAG | AGTTAGTCACGGACACCAG |
| CCL5 | Mouse | qPCR | CAAGTGCTCCAATCTTGCAGTC | TTCTCTGGGTTGGCACACAC |