FIGURE 1:

Native and exogenous condensates are nonrandomly localized. (A) Part of a large, multinucleate Ashbya cell. Ashbya grows in branched filaments called hyphae, each containing many nuclei residing in a common cytosol. Cells are made up of multiple hyphae and grow exclusively at regions called tips. Nuclei are labeled with HHF1-GFP. (B) Condensates comprised of endogenously expressed RNA-binding protein Whi3 localize near nuclei and at hyphal tips. (C) Exogenous synthetic phase-separating proteins are also largely located in the vicinity of nuclei and hyphal tips; 41.3% of hyphal tips had a condensate present within 1 μm of the tip apex, measured from the center of the condensate (N > 100 tips). In regions >1 μm from a tip, 72.5% of condensates were within 1 μm of a nucleus, measured from the center of the condensate to the edge of the nearest nucleus (N > 100 condensates).