Figure 7.

Representative kinetics of FRET (dimerization of T-cadherin) following the administration of 10 ug/mL adiponectin in HEK293 living cells. (a) The change in the normalized FRET compared to the baseline according to confocal laser scanning microscopy. Each point corresponds to the average NFRET value of a single microscopic image (a few thousand nonzero points). (b) The change in the compensated FRET signal measured by flow cytometry. Each point corresponds to the median fluorescence intensity in the 670/30 nm channel after 561 nm excitation of a few thousands living cells gated on the basis of forward and side light scattering. The solid lines represent the fitting of the data by exponential curves.