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. 2020 Jul 7;11:1525. doi: 10.3389/fmicb.2020.01525

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Copyright © 2020 Lemos, Florêncio, Pinto, Campos, Silva, Grazul, Pinto, Tavares, Scio, Apolônio, Melo and Fabri.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time-kill curve for C. tropicalis ATCC^® 28707 treated with different concentrations of scopoletin or fluconazole. Yeasts strain in BHI broth with scopoletin or fluconazole in different concentrations (MIC, MIC/2 and MIC/4) were evaluated by OD (530 nm) at 0, 2, 4, 6, 8, and 24 h of incubation at 35°C. Yeasts cultures with scopoletin vehicle served as growth control. The experiments were carried out in triplicate. Graphs were plotted as turbidity versus incubation time, and data represent the mean ± SEM.