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. 2020 Jul 7;11:1525. doi: 10.3389/fmicb.2020.01525

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Copyright © 2020 Lemos, Florêncio, Pinto, Campos, Silva, Grazul, Pinto, Tavares, Scio, Apolônio, Melo and Fabri.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of scopoletin treatment on cell density of C. tropicalis ATCC^® 28707. Compared with negative control (vehicle treatment), treatment with scopoletin induced a decrease in cell density similar to fluconozole treatment (positive control). Yeasts cells were stained with DAPI and counted under fluorescence microscopy. Representative images from DAPI-stained C. tropicalis are shown for each experimental group. Experiments were done in triplicate and data represent the mean ± SEM of yeast counted from 10 randomly selected fields/slide (n = 9 slides/time point). Letter (a) indicates statistically differences (ANOVA followed by Bonferroni, P < 0.05).