Figure 9. Latex bead tracking of monocytes in cochleae.

A. Image showing a cochlea examined at 3-days after noise exposure. The animal was injected with latex beads and its cochleae were stained with CD45 to illustrate immune cells. Monocyte-like CD45-positive cells lack latex beads (arrows). B. Image showing a cochlea from a Cx3cr1^GFP/+ mouse examined at 1-day after acoustic overstimulation. The cochlea was incubated with latex beads. Arrows point to GFP^l°^w cells that are latex bead-negative. Notice that GFP^high cells are latex bead-positive (inset). C. Image showing a cochlea examined at 20-days after noise exposure. The animal was injected with latex beads, and the cochlea was stained with CD45 to illustrate immune cells. The inset illustrates a latex bead-positive cell. n = 4 cochleae biological replicates for each group.