Figure 2.

T Cells Are Activated When Co-cultured with Autologous ER-Stressed iPSC-β

(A) Experimental design: PBMCs co-cultured with autologous iPSC-β.

(B–D) Flow cytometry data of T cells after a 4-8h co-culture with iPSC-β cells (n = 3 T1D and n = 1 ND donor, n = 3 differentiation batches per donor line). T1D¹, T1D², and T1D³ were pooled together.

(B) CD25⁺ and (C) CD69⁺ co-positive for CD3⁺, CD4⁺, or CD8⁺ cells, as indicated. The values are represented as adjusted MFI.

(D) Pro-inflammatory cytokine detection in supernatants collected after 48 h co-culture of PBMC with iPSC-β (n = 3 T1D and n = 1 ND donor, n = 3 differentiation batches per donor line). T1D¹, T1D², and T1D³ were pooled together.

(E) Percentage of live iPSC-β after co-culture, gated for C-peptide⁺/glucagon⁻ (n = 3 T1D and n = 1 ND donor, n = 3 differentiation batches per donor line). T1D¹, T1D², and T1D³ were pooled together.

^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.0005, and ^∗∗∗∗p < 0.0001. Ordinary 1-way ANOVA. ns, non-significant. See also Figure S2.