REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rat anti-C-peptide	Developmental Studies Hybridoma Bank (DHSB)	GN-ID4, RRID:AB_2255626
Mouse anti-NKX6.1	DHSB	F55A12, RRID:AB_532379
Mouse anti-glucagon	Santa Cruz Biotech	Cat#SC-514592, RRID:AB_2629431
Donkey anti-rat 594	Life Technologies	Cat#A21209, RRID:AB_2535795
Donkey anti-mouse Alexa 647	Life Technologies	Cat#A31571, RRID:AB_162542
Donkey anti-rabbit Alexa 488	Life Technologies	Cat#A21206, RRID:AB_2535792
Donkey anti-rabbit Alexa 594	Life Technologies	Cat#A21209, RRID:AB_2535795
Donkey anti-rabbit Alexa 647	Life Technologies	Cat#A31573, RRID:AB_2536183
Donkey anti-goat Alexa 647	Life Technologies	Cat#A21447, RRID:AB_141844
Donkey anti-sheep Alexa 488	Life Technologies	Cat#A11015, RRID:AB_141362
Donkey anti-rat 488	Jackson Laboratories	Cat#712-546-153, RRID:AB_2340686
Donkey anti-rat 405	Abcam	Cat#ab175670, RRID:AB_11009056
Mouse Anti-HLA-ABC PE-conjugated	Biolegend	W6/32, Cat#311406, RRID:AB_314875
Mouse anti-HLA-E PE-conjugated	Biolegend	3D12, Cat#342603, RRID:AB_1659250
Mouse anti-HLA-DR APC-conjugated	Biolegend	L243, Cat#307610, RRID:AB_314688
Mouse anti-PD-L1 APC-conjugated	Biolegend	29E.2A3, Cat#329708, RRID:AB_940360
Mouse anti-CD3 PB-conjugated	Biolegend	UCHT1, Cat#300417, RRID:AB_493094
Mouse anti-CD8 PE-conjugated	Biolegend	T8-Leu2, Cat#344705, RRID:AB_1953243
Mouse anti-CD4 PE/Cy7-conjugated	Biolegend	RPA-T4, Cat#300511, RRID:AB_314079
Mouse anti-CD69 Alexa 647-conjugated	Biolegend	FN50, Cat#310918, RRID:AB_528871
Mouse anti-CD25 Alexa 700-conjugated	Biolegend	M-A251, Cat#356118, RRID:AB_2562168
Mouse anti-CD95/Fas APC-conjugated	eBioscience	DX2, Cat#17-0959-42, RRID:AB_10807091
Mouse anti-CD49a PE-conjugated	BD Biosciences	Cat#559596, RRID:AB_397288
Mouse anti-CD26 APC-conjugated	Miltenyi Biotech	Cat# 130-120-769, RRID:AB_2752189
Mouse anti-Oct4 Alexa 488-conjugated	BD PharMingen	Cat#560253, RRID:AB_1645304
Mouse anti-Sox2 PE-conjugated	BD PharMingen	Cat#560291, RRID:AB_1645334
Mouse anti-SSEA4 V450-conjugated	BD PharMingen	Cat#561156, RRID:AB_10896140
Mouse anti-TRA-1-60 (A647-conjugated	BD PharMingen	Cat#560850, RRID:AB_10565983
Rabbit anti-PERK	Cell Signaling Tech.	D11A8, Cat#5683S, RRID:AB_10841299
Rabbit anti-PDI	Cell Signaling Tech.	C81H6, Cat#3501S, RRID:AB_2156433
Rabbit anti-BIP	Cell Signaling Tech.	C50B12, Cat#3177S, RRID:AB_2119845
Rabbit anti-GAPDH	Abcam	Cat#ab181602, RRID:AB_2630358
Bacterial and Virus Strains
lentiCRISPRv2	Broad institute	RRID: Addgene_52961
pHDM-vsvG, -tat, rev, gag/pol	Harvard Medical School DNA Resource Core	N/A
Sendai virus (SeV) Cytotune 2 kit	Life Technologies	Cat#A16517
Biological Samples
Human peripheral blood	University of Massachusetts Medical School	N/A
Human Peripheral Blood Leukapheresis Pack	Stem Cell Technologies	Cat#70500.1
Lenti-X 293T Cell Line	Takara Bio	Cat#632180
Chemicals, Peptides, and Recombinant Proteins
Activin A	R&D Systems	Cat#338-AC
Rock Inhibitor Y-27632	DNSK	Cat#DNSK-Kl15-02
Chir99021	Stemgent	Cat#04-0004-10
KGF	Peprotech	Cat#100-19
Retinoic acid	Sigma-Aldrich	Cat# R2625
LDN193189	Sigma-Aldrich	Cat#SML0559
Sant1	Sigma-Aldrich	Cat#S4572
PBDU	EMD Millipore	Cat#524390
XXI	EMD Millipore	Cat#565790
Alk5i II	Axxora	Cat#ALX-270-445
T3	EMD Millipore	Cat#642511
Betacellulin	ThermoFisher Scientific	Cat# 565790
Human IFN gamma	R&D Systems	Cat#285IF
Thapsigargin	Sigma-Aldrich	Cat#T9033
Critical Commercial Assays
MSD U-PLEX Metabolic group 1 kit	MesoScale Discovery	Cat# K151ACM
STEMdiff Cardiomyocyte Differentiation Kit	Stem Cell Technologies	Cat# #05010
Experimental Models: Cell Lines
Human iPSC-line T1D1	HSCI	iPSC 3107-001
Human iPSC-line T1D2	HSCI	iPSC 3107-002
Human iPSC-line T1D3	HSCI	iPSC 3107-003
Human iPSC-line ND	HSCI	iPSC 3107-013
Software and Algorithms
QuantStudio 6 Flex Real-Time PCR system	Applied Biosystems	N/A
FlowJo v10	BD (Becton, Dickinson and Company)	N/A
GraphPad Prism8	GraphPad Software	N/A
nCounter	NanoString technologies	N/A