Figure 3.

Western blots and BioNSi simulation analyses of IGF1R and INSR expression after KD. Western blots of IGF1R (A) and INSR (C) were conducted using total lysates of Control, IGF1R-KD and INSR-KD cells. One-hundred simulation steps were performed as described in Figure 2. A dashed vertical line indicates 50 steps of simulation. BioNSi plots of expression values against simulation steps are shown (B,D). Control (blue), IGF1R-KD (red), and INSR-KD (green). Splicing has occurred in the blot figures and full scans of the entire original (unprocessed) gels are presented in Supplementary Material. Squares in the uncropped films denote bands shown in the final figures.