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. 2020 Jul 7;11:435. doi: 10.3389/fendo.2020.00435

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Copyright © 2020 Sarfstein, Yeheskel, Sinai-Livne, Pasmanik-Chor and Werner.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Eight selected genes that do not change between control and KDs: BioNSi simulations validated by Western blots. Western blot analyses showing expression of HRas (A), TP53 (C), AKT3 (E), CASP3 (G), MTOR (I), PRKAA1 (K), p16 (M), and p21 (O) proteins were conducted on whole cell lysates of Control, IGF1R-KD, and INSR-KD cells. Hsp70 was used as a loading control. Data are representative of two independent experiments. (B,D,F,H,J,L,N,P) BioNSi plots of normalized expression values against 100 simulation steps in Control (blue), IGF1R-KD (red), and INSR-KD (green) cells. A dashed vertical line indicates 50 steps of simulation. Splicing has occurred in the blot figures and full scans of the entire original (unprocessed) gels are presented in Supplementary Material. Squares in the uncropped films denote bands shown in the final figures.