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. 2020 Jul 7;11:742. doi: 10.3389/fphys.2020.00742

FIGURE 1.

FIGURE 1

MiR-223 deficiency in diabetic platelet fails to suppress VSMC proliferation. Activated platelets (APs) from healthy subject (HS) and DM patients were cocultured with human VSMCs for 48 h. (A) Expression of differentiation markers ACTA2, CNN1, and TAGLN and dedifferentiation markers OPN, MYH10, KLF4, and KLF5 in control (Ctrl) VSMCs and APs cocultured with VSMCs (HS-APs, DM-APs) was detected with western blot analysis. (B,C) The rate of proliferation was determined by CCK8 assay and Brdu assay in VSMCs treated with APs pretreated by RNase A (degrades the RNA). Data are presented as mean ± SD (n = 6). Comparisons between more than two groups were analyzed using one-way ANOVA analysis with the Dunnett’s multiple correction. *P < 0.05, **P < 0.01, ***P < 0.001 vs Ctrl (VSMCs without platelets); #P < 0.05, ##P < 0.01, ###P < 0.001 vs HS-APs. (D) The rate of proliferation was determined by EdU incorporation assay in VSMCs transfected with miR-223 mimic. Data are presented as mean ± SD (n = 6). Comparisons between two groups were analyzed using unpaired nonparametric Student’s t-test.*P < 0.05 vs Ctrl (miR-NC).