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. 2020 Jul 7;11:742. doi: 10.3389/fphys.2020.00742

FIGURE 2.

FIGURE 2

MiR-223 directly targets the IGF-1R, which inhibits the phosphorylation of Akt and GSK3β. (A) Bioinformatics analysis identified a binding site for miR-223 in the 3′-UTR of IGF-1R. Luciferase activity assay in VSMCs following introduction of IGF-1R 3′ UTR or mutant 3′ UTR (mut) with or without miR-223 mimic (n = 3). VSMCs were transfected with miR-223 mimic for 48 h. (B,C) The expression of IGF-1R in VSMC was detected with western blot analysis. (D) The rate of proliferation was determined by CCK8 assay in VSMCs transfected with overexpression of IGF-1R. (E,F) The phosphorylation of Akt and GSK3β in VSMC was determined with western blot analysis. Data are presented as mean ± SD (n = 6). Comparisons between two groups were analyzed using unpaired nonparametric Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001 vs Ctrl (miR-NC).