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. 2020 Jul 7;11:742. doi: 10.3389/fphys.2020.00742

FIGURE 6.

FIGURE 6

Inhibition of calpain alleviates neointima formation by restoring the expression of miR-223 in diabetic platelet. (A) Immunofluorescence analysis of IGF-1R in uninjured or injured femoral arteries from Non-DM mice, and STZ-DM mice treated with AgomiR-NC or AgomiR-223 or calpeptin at 4 weeks after wire injury (n = 4). Green, IGF-1R; red, Acta2 blue; Dapi nuclear staining in VSMCs. Scale bars: 20 μm. (B) H&E staining of serial cross sections of femoral arteries from Non-DM mice, and STZ-DM mice treated with AgomiR-NC or AgomiR-223 or calpeptin at 4 weeks after wire injury (n = 4). Scale bars: 50 μm. (C) Quantification of IGF-1R expression in VSMCs in injured DM mice. (D) The intima size (I/M ration) in the injured femoral arterial sections has been quantified. (E) The miR-223 level in injured DM mice was detected by qRT-PCR assays. Data are presented as mean ± SD (n = 4). Comparisons between more than two groups were analyzed using one-way ANOVA analysis with the Dunnett’s multiple correction. *P < 0.05 vs non-DM; #P < 0.05, ##P < 0.01, ###P < 0.001 vs STZ-DM mice treated with AgomiR-NC.