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. 2020 Mar 9;13(4):718–726. doi: 10.1111/cts.12757

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© 2020 The Authors. Clinical and Translational Science published by Wiley Periodicals, Inc. on behalf of the American Society for Clinical Pharmacology and Therapeutics.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Schematic representation of sample preparation and analysis protocols. Proteins from plasma samples (10 µL) were precipitated with 0.1% trifluoroacetic acid (TFA) in isopropanol (IPA). Extracted proteins were reduced with dithiothreitol (DTT), alkylated with Iodoacetamide and digested with trypsin. Finally, digested samples were analyzed by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS). Prothrombin surrogate peptides from both glutamic acid (Gla)‐region and non‐Gla region (highlighted by blue color) were selected for the analysis.