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Assay performance. The linearity of the liquid chromatography‐tandem mass spectrometry assay was determined by spiking heavy peptide into human serum albumin (a‐ 2.5–100 µg/mL) and human serum matrix (b‐ 10, 40, and 80 µg/mL). Recovery was calculated by spiking the standard prothrombin at 40 and 80 µg/mL into pooled control serum samples (c).
