Table 2. Serology testing results from non-encephalitic acute febrile illness patients with confirmed JEV, probable JEV, DENV, and flavivirus infection.

No.	Patient sample ID	Age (years)	Days after onset	IgM		PRNT90 titer
				JEVa	DENVb	JEV	DENV-1	DENV-2	DENV-3	DENV-4
JEV (confirmed)
1	WGY599 S1	20	3	−	−	40	<10	<10	<10	<10
	WGY599 S2		6	+	+	1,280	20	80	20	20
2	WGY623 S1	32	3	−	−	<10	<10	<10	<10	<10
	WGY623 S2		7	+	+	640	40	40	80	80
3	WGY656 S1	23	2	EQU	−	20	<10	80	10	20
	WGY656 S2		3	+	−	>320	40	40	40	NA
4	WGY726 S1	32	7	+	+	80	10	40	80	10
	WGY726 S2		10	+	+	320	10	40	20	20
5	WGY733 S1	15	4	−	−	80	<10	40	80	20
	WGY733 S2		7	+	+	320	10	40	80	20
JEV (probable)
6	WGY067 S1	22	4	QNS	−	40	160	80	80	80
	WGY067 S2		7	+	−	80	>2,560	160	160	160
7	WGY143 S1	4	7	QNS	−	40	640	80	80	20
	WGY143 S2		12	EQU	−	160	>2,560	160	160	80
8	WGY317 S1	47	5	+	−	40	<10	80	10	40
	WGY317 S2		8	+	−	80	<10	160	10	80
9	WGY629 S1	19	5	+	−	160	20	160	80	160
	WGY629 S2		7	+	−	320	320	1,280	160	320
10	WGY647 S1	27	5	+	−	80	160	80	40	80
	WGY647 S2		6	+	−	160	1,280	20	160	80
11	WGY658 S1	30	3	EQU	−	<10	40	<10	<10	10
	WGY658 S2		5	EQU	−	80	80	<10	<10	<10
12	WGY667 S1	6	5	EQU	−	20	<10	20	10	40
	WGY667 S2		7	+	−	160	20	160	160	160
13	WGY713 S1	72	3	EQU	−	10	NA	40	NA	NA
	WGY713 S2		8	EQU	−	80	40	320	40	40
DENV
14	WGY065 S1	33	5	QNS	−	320	160	160	320	160
	WGY065 S2		8	+	+	320	>2,560	>2,560	>2,560	160
15	WGY071 S1	16	4	QNS	−	20	10	40	160	20
	WGY071 S2		7	+	+	20	80	160	>2,560	80
16	WGY321 S1	24	5	−	−	160	160	40	80	20
	WGY321 S2		7	+	+	160	1280	160	160	80
17	WGY724 S1	28	4	EQU	−	<10	40	10	20	10
	WGY724 S2		7	EQU	+	20	80	160	80	80
18	WGY727 S1	19	5	−	+	20	40	40	40	40
	WGY727 S2		6	EQU	+	160	>2,560	80	80	160
Flavivirus
19	WGY089 S1	33	5	QNS	+	40	20	80	80	40
	WGY089 S2		8	+	+	80	20	80	160	40
20	WGY174 S1	3	4	+	+	40	10	80	80	160
	WGY174 S2		5	+	+	320	10	320	80	320
21	WGY178 S1	20	3	+	−	80	40	80	<10	<10
	WGY178 S2		5	+	+	160	40	320	20	20
22	WGY516 S1	15	3	QNS	+	<10	<10	<10	40	<10
	WGY516 S2		5	EQU	+	20	<10	<10	40	<10
23	WGY575 S1	26	4	EQU	−	20	20	40	20	10
	WGY575 S2		5	+	+	160	40	80	160	40
24	WGY674 S1	21	5	−	−	40	<10	<10	<10	<10
	WGY674 S2		7	+	+	40	10	20	<10	20
25	WGY684 S1	16	5	+	+	80	<10	10	40	10
	WGY684 S2		6	+	+	80	10	20	40	20
26	WGY708 S1	31	3	+	−	NA	NA	NA	NA	NA
	WGY708 S2		6	+	+	40	40	40	20	40

^aRatios of samples optical densities divided by negative control were calculated; <2, negative (−); 2–3 equivocal (EQU); >3 positive (+).

^bCalculated ELISA binding index results ≥40 U were considered positive (+), while <40 U were considered negative (−).

S1, admission serum sample; S2, discharge serum sample; QNS, quantity not sufficient.