Pharmacological procedures (intervention and control)
Drug formulation
Dose
Volume
Concentration
Site and route of administration
Frequency of administration
Vehicle or carrier solution formulation and volume
Any evidence that the pharmacological agent used reaches the target tissue
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Cell lines
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Surgical procedures (including sham surgery)
Description of the surgical procedure
Anaesthetic used (including dose and other information listed in pharmacological procedures section above)
Pre- and postanalgesia regimen
Presurgery procedures (e.g., fasting)
Aseptic techniques
Monitoring (e.g., assessment of surgical anaesthetic plane)
Whether the procedure is terminal or not
Postsurgery procedures
Duration of the procedure and duration of anaesthesia
Physical variables measured
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Reagents (e.g., antibodies, chemicals)
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Pathogen infection (intervention and control)
Infectious agent
Dose load
Vehicle or carrier solution formulation and volume
Site and route of infection
Timing or frequency of infection
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Equipment and software
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Euthanasia
Method of euthanasia, including the humane standards the method complies with, such as the AVMA [125]
Pharmacological agent, if used (including dose and information listed in pharmacological procedures section above)
Any measures taken to reduce pain and distress before or during euthanasia
Timing of euthanasia
Tissues collected post-euthanasia and timing of collection
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