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. 2020 Jul 1;16(7):e1008883. doi: 10.1371/journal.pgen.1008883

Fig 3. UPB1 as a negative regulator in the BR signaling pathway.

Fig 3

(A, B) The root meristem size and cell number of 5-day-old Col-0, upb1-1, 35S::UPB1-HA-Flag#3, and 35S::UPB1-HA-Flag#8 seedlings in the absence or presence of different concentrations of BL for another day. (C, D) The root meristem size and cell number of 5-day-old Col-0, upb1-1, 35S::UPB1-HA-Flag#3, and 35S::UPB1-HA-Flag#8 seedlings in the absence or presence of different concentrations of BRZ for another day. (E) Phenotypes of 5-day-old seedlings of Col-0, upb1-1, det2-1, det2-1/upb1-1, bri1-5, bri1-5/upb1-1, BES1-RNAi, BES1-RNAi/upb1-1, bin2-1(+/-), bin2-1(+/-)/upb1-1, bin2-1(+/+), bin2-1(+/+)/upb1-1, bin2-3bil1bil2, and bin2-3bil1bil2/upb1-1. Bar = 0.5 cm. (F) Root meristem of Col-0, upb1-1, det2-1, det2-1/upb1-1, bri1-5, bri1-5/upb1-1, BES1-RNAi, BES1-RNAi/upb1-1, bin2-1(+/-), bin2-1(+/-)/upb1-1, bin2-1(+/+), bin2-1(+/+)/upb1-1, bin2-3bil1bil2, and bin2-3bil1bil2/upb1-1 in 5-day-old seedlings. White arrowheads (below) mark the position of the quiescent center (QC), and white arrowheads (above) mark the end of the meristem where cells start to elongate. Bar = 50 μm. The primary root length (G), meristem size (H), and meristem cell number (I) of the seedlings shown in (E). Date means ± SD (n≥20). Double asterisk represent highly significant differences (**, P<0.01; Student’s t test).