KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CD11b (MAC1) (M1/70) | BioLegend | Cat#: 101222; RRID: AB_493705 |
| CD117 (CKIT) (2B8) | BioLegend | Cat#: 105807; RRID: AB_313216 |
| GR1 (Ly6C/G) (RB6–8C5) | BioLegend | Cat#: 108431; RRID: AB_10896783 |
| CD48 (HM48–1) | BioLegend | Cat#: 103431; RRID: AB_2561462 |
| CD150 (TC15–12F12.2) | BioLegend | Cat#: 115914; RRID: AB_439797 |
| Sca-1 (D7) | BioLegend | Cat#: 108127; RRID: AB_10898327 |
| CD14 (HCD14) | BioLegend | Cat#: 325603; RRID: AB_830676 |
| TER119 (TER119) | BioLegend | Cat#: 116211; RRID: AB_313712 |
| O-GlcNAc (mouse monoclonal) | Cell Signaling Technology | Cat#: 9875S; RRID: AB_10950973 |
| Actin (rabbit monoclonal) | Cell Signaling Technology | Cat#: 4970S; RRID: AB_10694069 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Beta-estradiol | Sigma-Aldrich | E2758 |
| Polybrene | Millipore | TR-1003-G |
| Interleukin (IL)-3 | Peprotech | 200–03 |
| IL-6 | Peprotech | 200–06 |
| Fulvestrant | Sigma-Aldrich | I4409 |
| Leflunomide | Sigma-Aldrich | L5025 |
| Teriflunomide | Sigma-Aldrich | SML0936 |
| PuGNAc | Sigma-Aldrich | A7229 |
| Fibronectin (Human plasma) | Millipore | FC010 |
| 7-AAD | BD Pharmingen | 559925 |
| Propidium iodide | Life Technologies | P3566 |
| DAPI | Molecular Probes | D21490 |
| RNase A (PureLink) | Invitrogen | 12091021 |
| Uridine | Sigma-Aldrich | U3750 |
| DHODH (recombinant human protein) | Origene | TP309034 |
| Critical Commercial Assays | ||
| E. coli bioparticles (Molecular Probes) | Molecular Probes | E2861 |
| QIAGEN RNeasy Plus Mini Kit | QIAGEN | 74134 |
| CellTrace Far Red Cell Proliferation Kit | Thermo Fisher | C34564 |
| Wright-Giemsa | Siemens | 239020 |
| DNeasy Blood & Tissue Kit | QIAGEN | 69504 |
| LumiMax Superoxide Anion Detection Kit | Agilent Technologies | 204525 |
| Deposited Data | ||
| GEO: Gene expression of MLL/AF9 leukemia cells sorted from mice treated with or without brequinar | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84873 | GEO: GSE84873 |
| GEO: Time course of myeloid differentiation in the ysozyme-GFP ER-HoxA9 cells following estradiol withdrawal | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84874 | GEO: GSE84874 |
| GEO: Gene expression analysis of parental cell lines and cell lines with acquired resistance to compound 3 and compound 7 | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE84875 | GEO: GSE84875 |
| Experimental Models: Cell Lines | ||
| ER-HoxA9 | This paper | N/A |
| Lys-GFP-ER-HoxA9 | This paper | N/A |
| U937 | ATCC | CRL-1593.2 |
| THP1 | ATCC | TIB-202 |
| HL60 | ATCC | CCL-240 |
| MOLM13 | DSMZ | ACC 554 |
| Experimental Models: Organisms/Strains | ||
| Lysozyme2-GFP knockin mouse | MMRRC | B6.129(Cg)-Lyz2tm1.1Graf |
| C57BI/6J | Jackson Laboratories | 000664 |
| NOD/SCID (NOD.CB17-Prkdcscid) | Jackson | 001303 |
| Software and Algorithms | ||
| FlowJo, version 10 | FlowJo | N/A |
| ModFit LT, version 4 | Verity | N/A |