Figure 2. PG-1 induced leakage of anionic bilayers.
The leakage of ANTS/DPX probes encapsulated in 50POPG:50POPC LUV was measured before and after the addition of PG-1. The kinetics of the leakage event were dependent on [peptide], with samples containing 1:200 peptide:lipid ratios showing a fast saturation at ~60% leakage. The reduced state of the peptide, controlled by the addition of 2-mercapthoethanol (ME) indicated in orange, did not affect leakage kinetics.