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. Author manuscript; available in PMC: 2021 Apr 17.
Published in final edited form as: ACS Chem Biol. 2020 Mar 18;15(4):849–855. doi: 10.1021/acschembio.0c00036

Figure 3.

Figure 3.

Cleaving capacity and selectivity of small-molecule cleavers. (A) Binding affinity of 1 for r(CUG)12 (Kd = 610 ± 150 nM), r(GC)8 (Kd > 20 μM), and DNA (Kd > 20 μM); n = 3. (B) Quantification of cleavage of r(CUG)10 by 1, 2, Cugamycin, BLM, and DeglycoBLM; n = 3. (C) Quantification of cleavage of DNA by 1, 2, Cugamycin, BLM, and DeglycoBLM; n = 3. (D) Effects of 1, Cugamycin, BLM, and DeglycoBLM on γ-H2AX, a marker of DNA damage, in C2C12 cells. n = 8, (**) P < 0.01, (***) P < 0.001, as determined by comparison to untreated cells by a one-way analysis of variance (ANOVA). Error bars indicate the standard deviation (SD) for all panels.