Fig. 1.
Histology, morphology: ( A ) hematoxylin and eosin (H&E) staining showing histology of mouse lungs in room air (RA), bronchopulmonary dysplasia (BPD) (100% O 2 from postnatal or PN day 0-PN4; recovery in RA for next 10 days till PN14), (RA + AVR-25) and (BPD + AVR-25) treated groups. In the BPD group, the alveoli are larger and simplified as compared with RA controls. After treatment with AVR-25 (10 mg/kg), intraperitoneally (IP) (30 µL), at PN2 and PN4, the alveolar architecture recovers, in part, similar to those of RA controls. There was no change in the histology in the (RA + AVR-25) treated group. Bottom panel shows improvement in ( B ) chord length, ( C ) septal thickness and ( D ) radial alveolar counts or radial alveolar count (RAC) after treatment. (X100). * p < 0.05, *** p < 0.0001, one-way analysis of variance (ANOVA), n = 3–6.