Table 1.
Clinical parameters and data
| Day 1 | Day 4 | Day 7 | Day 8 | Day 9 | Day 11 | Day 14 | |
|---|---|---|---|---|---|---|---|
| Event | Admission | AKI | CRRT | ||||
| sCre, mg/dL | 1.34 | 1.34 | 1.25 | 2.08 | 3.46 | 4.52 | 6.21 |
| Urine volume, mL/day | 2,240 | 864 | 558 | 398 | 20 | 7 | |
| SOFA score | 11 | 13 | 14 | 15 | 21 | 15 |
| Results of RT-PCR for SARS-CoV-2 (Ct) |
|||||||
|---|---|---|---|---|---|---|---|
| Day 1 | Day 3 | Day 5 | Day 7 | Day 9 | Day 11 | Day 14 | |
| Blood | 268.0 | 316.4 | 68.9 | 25.4 | UD | UD | |
| (34.73) | (34.48) | (36.78) | (38.35) | ||||
| Pharyngeal swab | 1,766.0 | 384.1 | 20.5 | ||||
| (31.88) | (31.4) | (35.93) | |||||
| Lower respiratory tract | 1,379,500.0 | 48,200.0 | 4,586.0 | 466.7 | 1,254.5 | ||
| (21.81) | (26.88) | (30.43) | (31.11) | (29.57) | |||
| CRRT effluent | 1 h; 19.21 (36.05) | 1 h; UD | |||||
| 3 h; UD | 3 h; 12.63 (37.45) | ||||||
| 6 h; 20.72 (35.91) | |||||||
CRRT effluent samples were taken on day 11 (1, 3, and 6 h after CRRT start) and on day 14 (1 and 3 h after CRRT start). Data are presented as virus copies per 1 µL (Ct). Lower cycle threshold (Ct) values indicate higher viral loads. RT-PCR was performed using Quantitect probe one-step RT-PCR kit (Qiagen, MD, USA) with the following probe and primer sets; WuhanCoV-spk2-f 5′-TTTCCTCGTGAAGGTGTCTTTGT-3′, WuhanCoV-spk2-r 5′-TGTGGTTCATAAAAATTCCTTTGTG-3′, and WuhanCoV-spk2-hex-p 5′-HEX-TCAAATG-GCACACACTGGTTTGT-BHQ1 targeting spike gene (24,843–24,916 in GenBank accession MN908947); WuhanCoV-N1f 5′-GGCCGCAAATTGCACAAT-3′, WuhanCoV-N1r 5′-CCAATGCGCGACATTCC-3′, and WuhanCoV-N1pr-fam 5′-FAM-CCCCCAGCGCTTCAGCGTTCT-TAMRA-3′ targeting nucleoprotein gene (29,191–29,251 in MN908947). SOFA, sequential organ failure assessment; CRRT, continuous renal replacement therapy; AKI, acute kidney injury; UD, undetected.