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. 2020 Jul 14;10:11598. doi: 10.1038/s41598-020-68179-9

Figure 1.

Figure 1

Biofilm formation by Bifidobacterium breve UCC2003 under different stress conditions (A). Biofilm was allowed to form for 24 h under various conditions including pH 4–6.8, sucrose 1.46 mM to 58 mM, NaCl 94.5 mM to 426 mM and porcine bile 0.05% to 2% (w/v). The pH of RCM was 6.8. Biofilm formation was assessed by crystal violet staining with absorbance read at O.D.570 nm. Biofilm formation by several species/strains of bifidobacteria (B). Biofilm induced by addition of 0.5% or 1% (w/v) of porcine bile and allowed to form for 24 h. Biofilm was stained with crystal violet and the absorbance read at O.D.570 nm. Negative controls with just RCM (non-inducing biofilm conditions) were also included for each species. Experiments were carried out in triplicate and error bars represent standard deviation.