Figure 3.

Biofilm formation by mutants screened from a Bifidobacterium breve UCC2003 transposon mutant bank. A transposon mutant bank was screened using the crystal violet assay. Biofilm was induced with 0.5% (w/v) porcine bile and allowed to form for 24 h. An insertional mutant B. breve UCC2003 luxS, and EPS deficient strain, B. breve UCC2003 ${\text{EPS}}^{-}$, were also screened. A mutant in biofilm formation was assumed to have reduced biofilm biomass, as compared to the wildtype, due to reduced absorbance at O.D._570 nm. Experiments were carried out in triplicate and error bars represent standard error of the mean.