Figure 6.

Dispersal of mature biofilms of Bifidobacterium breve UCC2003 wildtype (WT) and a B. breve UCC2003 derivative deficient in exopolysaccharide production (EPS⁻). Biofilm was induced by supplementation of media with 0.5% (w/v) porcine bile and biofilms were allowed to form for 24 h. Mature biofilms were then treated with DNaseI (10 U/ml) in 5 mM MgCl₂ and 0.95 mg/ml proteinase K in 20 mM in Tris–HCl. Biofilms were stained with crystal violet and absorbance read at ${\text{O.D.}}_{\text{570nm}}$. Biofilm formation of B. breve UCC2003 WT was taken to be 100%. All experiments were carried out in triplicate and error bars represent standard deviations.