Table 2.

Transposon insertions isolated in crystal violet biofilm screen.

Mutant	Gene locus	Function
Bbr_1202*	oppD2/oppB2/oppC1operon	Oligopeptide transporter
Bbr_1738	dapE	Succinyl-diaminopimelate desuccinylase, lysine and cell wall synthesis
Bbr_1901	nrdH, nrdI, nrdE operon	Ribonucleotide reductase
Bbr_0074	pepX	Xaa –Prolyl Peptidase
Bbr_1719/20/21†	accC/accD/fas operon	Fatty acid biosynthesis
Bbr_200	NADH Flavin reductase	DNA binding protein/NADH Flavin reductase
Bbr_201	DNA binding protein	DNA binding protein/AAA ATPase
Bbr_1654/53/52/51	serA2	Non-functional conserved protein/Phosphoglycerate dehydrogenase/Thymidlate synthase
Bbr_0060	glgP1	Glycogen phosphorylase
Bbr_1353	proP	Osmolarity/stress MFS
Bbr_1580	Conserved Hypothetical Protein	Transmembrane protein/hydrolase

*Gene was isolated twice in mutant screen. (Distinct mutants in the same gene).

^†Gene was isolated three times in mutant screen. (Distinct mutants in the same gene).