Table 4.
Oligonucleotides used in this study.
| Primer | Function | Sequence |
|---|---|---|
| iPCR-Fw | Forward primer for inverse PCR reaction | GCATACCGTACTGATCTG |
| iPCR-Rv | Reverse primer for inverse PCR reaction | CAATCATACCGGCTTCC |
| Arb6 | Primer for arbitrary PCR | GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC |
| Arb2 | Primer for arbitrary PCR | GGCCACGCGTCGACTTAGTTAC |
| Arb1 | Primer for arbitrary PCR | GGCCACGCGTCGACTAGTTACNNNNNNNNNNGATAT |
| TnTetL1 | Primer for arbitrary PCR | AAAACATGGTGTCCGTCCTC |
| TnTetR1 | Primer for arbitrary PCR | TCGCTGGGATACTTGAACCA |
| TnTetL2 | Primer for arbitrary PCR | GCTGTGGTGTTTGGTTGGAA |
| TnTetR2 | Primer for arbitrary PCR | CTCTATGCGCCCCAGGAATA |
| pMod-Fw-seq | Forward sequencing primer based on transposon | GCCAACGACTACGCACTAGCC |
| pMod-Rv-seq | Reverse sequencing primer based on transposon | GAGCCAATATGCGAGAACACC |