Table 1.

Isolated RNA from randomly selected 20 boar spermatozoa.

Sample name	Litter size	Isolated RNA without DNase		Isolated RNA with DNase
		Concentration (ng/µL)	260/280 ratio	Concentration (ng/µL)	260/280 ratio
1	14.15 ± 0.79	41.25	1.74	67.72	1.86
2	13.44 ± 0.68	153.97	1.86	35.80	1.79
3	13.30 ± 0.95	75.89	1.74	74.12	1.93
4	13.15 ± 0.79	140.45	1.87	123.00	1.85
5	13.14 ± 0.55	146.45	1.87	58.04	1.86
6	13.10 ± 0.99	88.29	1.83	17.00	1.54
7	12.90 ± 1.06	152.45	1.85	28.12	1.81
8	12.82 ± 0.80	25.81	1.70	129.56	1.93
9	12.79 ± 0.94	109.57	1.79	96.12	1.91
10	12.75 ± 1.28	197.57	1.82	21.40	1.89
11	12.67 ± 0.72	255.65	1.83	13.40	1.61
12	12.65 ± 0.64	29.33	1.77	21.08	1.84
13	12.54 ± 0.75	58.93	1.83	42.84	1.84
14	12.40 ± 1.72	93.89	1.82	33.64	1.88
15	12.38 ± 0.85	33.89	1.58	10.12	1.80
16	12.25 ± 0.77	83.89	1.82	33.96	1.84
17	12.08 ± 0.51	108.29	1.80	37.32	1.77
18	11.81 ± 0.71	54.45	1.80	18.28	1.55
19	11.75 ± 1.16	128.37	1.85	13.64	1.55
20	11.35 ± 0.92	56.37	1.78	10.36	1.72
Average	12.67 ± 0.14	101.7 ± 13.5*	1.80 ± 0.01	44.28 ± 8.12	1.79 ± 0.03

The male fertility data (total litter size) and RNA quantity and quality of isolated RNA with or without DNase in 20 randomly selected boar spermatozoa. *P < 0.05, calculated using two-tailed Student's t-test.