Table 1.
Top most differentially expressed genes in primary insulinoma vs normal pancreas. In bold genes used for validation with qRT-PCR.
| Gene | Gene symbol | Log2FC | P-value | FDR | Role |
|---|---|---|---|---|---|
| Pancreatic duodenal homeobox 1 | PDX1 | 2.13 | 0.0001 | 0.003 | Beta-cell differentiation |
| Paired homeobox 4 | PAX4 | 6.23 | 3.32E−09 | 3.92E−06 | |
| Insulinoma associated 1 | INSM1 | 5.021 | 3.40E−07 | 5.17E−05 | |
| NK2 homeobox 2 | NKX2 | 5.32 | 3.40E−09 | 3.92E−09 | |
| Nestin | NES | 2.53 | 0.0001 | 0.003 | |
| Delta/notch-like EGF repeating containing ligand | DNER | 4.41 | 3.05E−07 | 4.83E−05 | Pancreatic ontogeny |
| SRY-box 17 | SOX17 | 2.20 | 0.009 | 0.04 | |
| SRY-box 18 | SOX18 | 2.89 | 0.003 | 0.02 | |
| Hes-related family BHLH transcription factor 1 | HEY1 | 2.46 | 0.0009 | 0.01 | |
| Glutathione peroxidase 3 | GPX3 | 4.73 | 3.91E−08 | 1.42E−05 | Glucose metabolism |
| Glucokinase | GCK | 5.37 | 3.13E−08 | 1.23E−05 | |
| Solute carrier 38 family 8 | SCL38F8 | 9.65 | 9.63E−08 | 2.35E−08 | Insulin secretion |
| Calcium bynding protein 1 | CA1 | 5.09 | 1.66E−08 | 8.82E−06 | |
| Potassium voltage-gated channel subfamily H member 2 | KCNH2 | 5.47 | 7.40E−09 | 6.63E−06 | |
| Otoferlin (calcium sensor) | OTOF | 5.56 | 9.07E−08 | 2.32E−05 | |
| Tetraspanin 1 | TSPAN1 | 5.46 | 5.95E−08 | 1.97E−05 | Insulin production |
| Insulin-degrading enzyme | IDE | − 2.29 | 7.33E−06 | 0.0003 | |
| Insulin growth factor receptor 2 | IGF2 | 4.33 | 9.30E−06 | 0.0004 | |
| Insulin like 6 | INS | 3.21 | 0.007 | 0.04 | |
| Insulin receptor | INSR | − 2.62 | 1.00E−07 | 2.35E−05 | |
| Islet amyloyde polyptide | IAPP | 4.37 | 2.32E−06 | 0.0001 | Amyloid deposition |
| Chromogranin B | CHGB | 4.58 | 1.33E−07 | 2.79E−05 | Pancreatic neuroendocrine tumours markers |
| Secretogranin II | SCG2 | 4.97 | 1.11E−07 | 2.49E−05 | |
| Synapsin I | SYN1 | 5.59 | 7.87E−09 | 6.68E−06 | |
| Alpha amylase | AMY1A | − 7.63 | 8.93E−09 | 6.88E−06 | Pancreatic exocrine marker |
| Pseudopodium-enriched atypical kinase 1 | PEAK1 | − 3.09 | 6.10E−09 | 5.78E−06 | Glucose metabolism |
| Solute carrier 7 family 1 | SLC7A1 | − 3.03 | 2.37E−07 | 4.13E−05 | |
| Serpin inhibitor peptidase clade I | SERPINA1 | 3.93 | 2.08E−07 | 3.87E−05 | Ductal cell markers |
| Pappalysin 2 | PAPPA2 | 5.40 | 1.00E−07 | 2.35E−05 | |
| Glucagon receptor | GCGR | 7.63 | 3.54E−10 | 1.30E−06 | Alpha cell marker |
| Integrin-alpha 2 (CD49b) | ITA2 | − 3.45 | 2.76E−08 | 1.17E−05 | Cell adhesion |