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. 2020 Jul 14;10:11608. doi: 10.1038/s41598-020-68406-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparative expression of CFIm25 vs involved miRNAs. (a) CFIm25 expression level in samples acquired from breast cancer patients and control group. CFIm25 is significantly lower in patients compared to controls. (b) Relative gene expression of miRNAs in patients. (c) Distribution of miRNA expression in patients according to low and high expression levels of CFIm25. Patients categorized as low or high CFIm25‑expressing tumors, using median expression of CFIm25 in normal samples as a cut-off. Data represent mean of at least three independent experiments. The star above the bars, represents significant difference respect to the corresponding control reference. The results are relative gene expression after normalization with β-actin for CFIm25 and SNORD47 for miRNA genes, using 2^−ΔΔCt method.