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. 2020 Jul 14;10:11608. doi: 10.1038/s41598-020-68406-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Some of predicted miRNAs directly regulate CFIm25 expression. (a) CFIm25 expression level in cell line with enforced expressions of miRNAs of interest, monitored by Real-time PCR and (b) Western blot technique. The western blot bonds cropped from a same gel and membrane. The original image of the membrane and gel could be found in supplementary Figs. 1 and 2. (c) Luciferase assay of the HEK293 cells that are transiently transfected with CFIm25-pSICHECK2 construct and WT-miRNAs/mut-miRNAs. (d) Expression level of CFIm25 in cells transducted with either of miR-Off-23 or miR-Off-374 constructs. Protein normalization performed against β-actin expression. Columns are mean of three different experiments and error bars are standard deviation (p < 0.05). The star represents significant difference respect to the corresponding control reference.