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. 2020 Jul 14;10:11608. doi: 10.1038/s41598-020-68406-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Viability test of MDA-MB-231 cells that were transducted with different cloned miRNAs. (a) MTT assay of MDA-MB-231 cells that are transducted with miRNAs of interest. MTT assay of transducted MDA-MB-231 cells by (b) miR-Off-23 and (c) miR-Off-374 that transfected by shCFIm25 vector. Each time point was expressed as total absorbance at 570 nm after background subtraction (Y axis). Points are mean of three experiments and error bars represent standard deviation. (d) CFIm25 expression level in shCFIm25 transfected cells. The star above the bar, represents the significant difference respect to the corresponding control reference.