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. 2020 Jul 14;10:11538. doi: 10.1038/s41598-020-67999-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A Maximum Likelihood tree constructed from the RAD-seq of the non-recombining region in 12 native S. invicta and S. richteri samples. The two S. invicta reference genomes (marked INVB-ref and INVb-ref) are also included. The monophyletic subtree of the Sb haplotypes is marked by a blue box. The homologous sequence in S. fugax genome was used as an outgroup to root the tree but is not shown.