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. 2020 Jul 14;10:11559. doi: 10.1038/s41598-020-68508-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histology at 180 days post-exposure. Glycan images showing increases in relative intensity specific to distinct pathologies in the IR lungs (bottom images in each panel) compared to the control samples (top images in each panel). Top images in each panel are control, animal ID numbers as shown left to right: 0147, 0879, 08094011. Bottom images in each panel are IR, animal ID numbers as shown left to right: 04923, R03007, 040087. Changes were more pronounced in the sample from 04923, bottom left of each panel. Glycan structures were detected as the [M + Na] + ion and tentatively assigned. Hex = Hexose, dHex = fucose and HexNAc = N-acetylhexosamine.