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. 2020 Jul 14;10:11559. doi: 10.1038/s41598-020-68508-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MSI and mass spectra at 180 days post-exposure. MALDI-MS merged image of two high mannose glycan structures, tentatively assigned as Hex3HexNac5 and Hex7HexNac2, showing differential distribution in a lung sample taken at 180 days post-radiation (from 04923) in (A). The H&E stained section is shown in (B) and the MSI overlaid with the H&E section is shown in (C). Single pixel spectra and higher magnification stained sections are shown for regions of mucin accumulation (D) and blue boxes, and regions of high accumulation of alveolar macrophages (E) and red boxes.