Table 2.
Antibodies used for Western blotting (WB) and immunohistochemistry (IHC)
| Antibody | Application | Company | Reference | Dilution |
|---|---|---|---|---|
| α-Tubulin (mouse) | WB | Sigma-Aldrich | T6199 | 1:500 |
| β-Actin (mouse) | WB | Santa Cruz | sc-69879 | 1:500 |
| VE-cadherin (goat) | WB/IHC | Santa Cruz | sc-6458 | 1:200 |
| Claudin-5 (mouse) | WB/IHC | Invitrogen | 352500 | 1:200 |
| CD31 (rat) | IHC | Dianova | DIA310 | 1:50 |
| Occludin (mouse) | IHC | Invitrogen | 331500 | 1:200 |
| ZO-1 (rabbit) | IHC | Invitrogen | 617300 | 1:200 |
| Podocalyxin (goat) | IHC | R&D Systems | AF1556 | 1:200 |
| HIF-1α (rabbit) | WB/IHC | Novus Biologicals | NB100-134 | 1:200 |
| Pneumococcal (rabbit) | IHC | Dr. Hammerschmidt | NA | 1:500 |
| Donkey anti-goat 555 | IHC | ThermoFisher | SA5-10087 | 1:500 |
| Donkey anti-rabbit 488 | IHC | ThermoFisher | SA5-10038 | 1:500 |
| Donkey anti-mouse 555 | IHC | ThermoFisher | SA5-10167 | 1:500 |
| Donkey anti-mouse 680 | WB | LI-COR | 925-68072 | 1:2000 |
| Donkey anti-rabbit 800 | WB | LI-COR | 925-32213 | 1:2000 |
| Donkey anti-goat 800 | WB | LI-COR | 925-32214 | 1:2000 |