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. 2020 Jul 8;8:566. doi: 10.3389/fcell.2020.00566

FIGURE 3.

FIGURE 3

Overexpression of HIF-2α P405/531A [HIF-2α(mt)] inhibits the expression of fructolytic genes in the liver. (A) Expression of fructolytic genes and splicing factors in livers from 8-week-old Atg7f/f mice infected with 3 × 109 pfu of Ad-GFP (control) or different amounts of pfu of Ad-HIF-2α(mt) [Ad-GFP: n = 6; 3 × 109 pfu Ad-HIF-2α(mt): n = 7; 1 × 109, 5 × 108, 1 × 108 pfu Ad-HIF-2α(mt): n = 3–4]. Each value represents the amount of mRNA relative to that in GFP-infected mice. Cyclophilin was used as the invariant control. (B) Immunoblots of liver lysates with antibodies against total KHK, ALDOB, splicing factors (A1CF, HNRNPH1/2, and SF3B1) and γ-tubulin as loading control. (C) Quantification of KHK and ALDOB immunoblots after normalization with γ-tubulin. Data are mean ± SD. Statistical analysis was performed using one-way ANOVA followed by Dunnett’s multiple comparisons test. *p < 0.05; **p < 0.01; ***p < 0.001 vs. GFP-infected mice.