FIGURE 6.

Adhesion and invasion of CDprotect isolate ZvL2 and K-12 MG1655 laboratory strain to intestinal epithelial cells Caco-2. ZvL2, Mg1655—after one passage and ZvL2 Glu, Mg1655 Glu—after five passages from colony on minimal M9 medium supplemented with glucose, ZvL2 PA, Mg1655 PA—after five passages from colony in minimal M9 medium supplemented with sodium propionate. ZvL2 PA-Glu—re-passaging on M9 medium supplemented with glucose after sodium propionate. The kinetics of adhesion and invasion were measured up to 3 and 5 h at an MOI of 10. Adhesion and invasion were also represented as a percentage of initial inoculums. Data are expressed as the means ± SD from three independent experiments. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.