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. 2020 Jul 8;8:549. doi: 10.3389/fcell.2020.00549

FIGURE 2.

FIGURE 2

Functional modulation of TRPM7 channels by EDPs. (A) Effect of EDPs incubation on TRPM7 expression assessed by qRT-PCR in MIA PaCa-2 cells. (B) Effect of TRPM7 silencing on TRPM7 expression assessed by qRT-PCR. (C) Typical example of lysates from MIA PaCa-2 cells transfected with a scrambled siRNA (siControl) or targeting TRPM7 (siTRPM7) and immunoblotted with anti-TRPM7 and anti-GADPH antibodies. (D) Quantification of immunoblotting normalized to siControl. (E) Typical example of MIC current recorded at +100 mV (outward current) and at −100 mV (inward current) before (1), during (2), and after (3) the application of AG-9 (10–7 M). (F) Current–voltage relationships corresponding to the traces recorded in (C). (G) Averaged current–voltage relationship of AG-9-activated currents recorded in cells transfected with a non-targeting siRNA (siControl, black traces) and in cells transfected with a siRNA targeting TRPM7 (siTRPM7, red traces). (H) Current densities of AG-9-activated currents recorded at +100 mV. *P < 0.05 and **P < 0.01 by Mann-Whitney rank sum tests.