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. 2020 Jul 14;10:11597. doi: 10.1038/s41598-020-68396-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Semi-quantitative assessment of intensity of PAS reaction. (A) Example from a subject age < 35 years, (B) example from a subject > 50 years, arrows indicating signal from PAS and hematoxylin stained areas. (C) Results of the analysis in 6 human subjects, 3 younger (< 35 years) and 3 older (> 50 years). In subjects > 50 years, the average glycogen content was significantly higher than that found in human subjects < 35 years. The difference is statistically significant (one sided t-test, p = 1.2 × 10^–6, N ≥ 15 CD34+ cells for each individual subject. Software: Fiji plug-in Color Inspector 3D.