Skip to main content
. 2020 Jul 14;11:3516. doi: 10.1038/s41467-020-17091-x

Fig. 7. Loss of IRSp53 alters the opposing plasma membranes at the nascent AMIS.

Fig. 7

a MDCK Ctr (top) and IRSp53-KO cells (middle) were seeded as single cells on Matrigel-coated gridded coverslips. The cysts were fixed 16 h after seeding, stained with anti-ß-catenin (green) and DAPI (blue). Two-cell stage cysts were initially identified on grids by confocal microscopy. Scale bar, 10 µm. Samples were then processed for electron microscopy, with images of the corresponding cells shown at the indicated magnifications. Arrowheads, inter-cytoplasmic bridges of the plasma membranes along the AMIS. Bottom: Quantification of the inter-cytoplasmic bridges/µm. Two-cell stage cysts were analysed from n = 2 independent experiments. In all, n = 484 (Ctr) and n = 332 (KO) different fields from n = 6 (Ctr; KO) samples were counted from serial sections along the Z-axis. Data are means ± s.e.m. P value, student’s t-test two-tailed. ***p < 0.001. Source data are provided as a Source Data file. b Images of a three-dimensional tomographic reconstruction of an inter-cytoplasmic bridge at the opposing membranes of MDCK IRSp53-KO cells during early cystogenesis (see also Supplementary Movie 4). Arrowheads, inter-cytoplasmic bridge.