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. 2020 Jul 14;10:11586. doi: 10.1038/s41598-020-68548-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ISR bioassay for the sub-fraction of S11 by Sephadex LH-20 column chromatography. The soybean seeds treated with S11-1, S11-2, S11-3, S11-4, S11-5, S11-6 sub-fraction and control (2%DMSO) at 12 dpi inoculation. (A) Percentage of various stage nematodes. The ratio of different developmental stages of SCN to the total number of nematodes within soybean roots. (B) Number of nematodes per plant. The different lowercase letters on the bars represent significant differences (P < 0.05) between treatments. The data are means ± SE. n = 3.