FIGURE 2.

SeMet inhibited activation of TLR4/NF-κB pathway induced by ESBL-E. coli in bMECs. bMECs were pre-treated with various concentrations of SeMet for 12 h, followed by ESBL-E. coli for 4 h. (A–G) Protein levels of SeS, TLR4, IKB, NF-κB, p-p65 (Ser536), TNF-α, and IL-1β were determined by Western blotting. GAPDH was used as loading controls. Data represent means ± SD of three independent experiments. In each independent experiment, there were 10 replicates per group and each sample was assayed in duplicate with similar results. “–” after Se and SeMet indicated that SeMet was not added. “–” and “+” after E. coli indicated that E. coli (MOI = 5) were not or were added, respectively. *p < 0.05 and **p < 0.01, differences compared to control group; ^##p < 0.01 differences compared to ESBL E. coli-infected samples.