FIGURE 3.

SeMet reversed activation of TLR4/NF-κB pathway induced by ESBL-E. coli in macrophages. Macrophages were pre-treated with various concentrations of SeMet for 12 h, followed by ESBL-E. coli for 6 h. (A–G) Protein levels of SeS, TLR4, IκB, NF-κB, p-p65 (Ser536), TNF-α, and IL-1β were determined by Western blotting. GAPDH was used as loading controls. Data represent means ± SD of three independent experiments. In each independent experiment, there were two replicates per group with similar results. “–” after Se and SeMet indicated that SeMet was not added. “–” and “+” after E. coli indicated that E. coli (MOI = 5) were not or were added, respectively. **p < 0.01, differences compared to the control group; ^#p < 0.05 and ^##p < 0.01 differences compared to ESBL E. coli-infected samples.