TABLE 1.
Primer sequences used for real-time PCR.
| Target gene | Oligonucleotide sequence (5’ to 3’) | Annealing temperature (°C) | Product size (bp) |
| 18S | Fw: GTAACCCGTTGAACCCCATT Rv: CCATCCAATCGGTAGTAGCG | 57 | 151 |
| COL2A1 | Fw: AGGGCCAGGATGTCCGGCA Rv: GGGTCCCAGGTTCTCCATCT | 57 | 195 |
| ACAN | Fw: CAACTACCCGGCCATCC Rv: GATGGCTCTGTAATGGAACAC | 56 | 160 |
| COL1A1 | Fw: TCCAACGAGATCGAGATCC Rv: AAGCCGAATTCCTGGTCT | 57 | 191 |
| SOX9 | Fw: CCCAACGCCATCTTCAAGG Rv: CTGCTCAGCTCGCCGATGT | 60 | 242 |
| ACTA2 | Fw: ATGCCATCATGCGTCTGGAT Rv: ACGCTCAGCAGTAGTAACGA | 60 | 101 |
| NOTCH1 | Fw: AAGCTGCATCCAGAGGCAAAC Rv: TGGCATACACACTCCGAGAACAC | 60 | 172 |
| CDH2 | Fw: GCGTCTGTAGAGGCTTCTGG Rv: GCCACTTGCCACTTTTCCTG | 60 | 293 |
Forward (Fw) and reverse (Rev) primers. COL2A1, collagen type II alpha 1 chain; ACAN, aggrecan; COL1A1, collagen type I alpha 1 chain; SOX9, SRY-box transcription factor 9; ACTA2, actin alpha 2, smooth muscle; NOTCH1, notch receptor 1; CDH2, cadherin 2.